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c jun gel supershift antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology c jun gel supershift antibody
    C Jun Gel Supershift Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+jun+gel+supershift+antibody/c-Jun+Antibody/pmc04802467-60-9-16
    Average 96 stars, based on 2667 article reviews
    c jun gel supershift antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Varicose Remodeling of Veins Is Suppressed by 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase Inhibitors
    Article Snippet: .. To ascertain the binding specificity, 2.0 μL of a c‐jun gel supershift antibody (2 mg/mL, sc‐1694; Santa Cruz Biotechnology) per 6.0 μL of nuclear extract (10 μg protein) was added 30 minutes before addition of the labeled oligonucleotide probe and incubated for another 30 minutes at room temperature. .. Total RNA was isolated from cultured cells by solid phase extraction with the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions. cDNA was synthesized using the Omniscript RT Kit (Qiagen), according to the manufacturer's protocol.

    Article Title: Varicose Remodeling of Veins Is Suppressed by 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase Inhibitors
    Article Snippet: Preparation of nuclear extracts from the cultured HUVSMCs and subsequent nondenaturing 4% polyacrylamide gel electrophoresis were carried out, as described previously.15 The double-stranded gel shift oligodeoxynucleotide for AP-1 (50-GTGCTGACTCAGCAC-30) was end-labeled with [c-32P]ATP by using the 50-end labeling kit from Amersham GE Healthcare. .. The double-stranded AP-1 oligodeoxynucleotide was prepared from complementary single-stranded bonded oligodeoxynu- cleotides obtained from Biomers, as described prevously.16 To ascertain the binding specificity, 2.0 lL of a c-jun gel supershift antibody (2 mg/mL, sc-1694; Santa Cruz Biotechnology) per 6.0 lL of nuclear extract (10 lg protein) was added 30 minutes before addition of the labeled oligonucleotide probe and incubated for another 30 minutes at room temperature. .. Total RNA was isolated from cultured cells by solid phase extraction with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. cDNA was synthesized using the Omniscript RT Kit (Qiagen), according to the manufacturer’s protocol.

    Labeling:

    Article Title: Varicose Remodeling of Veins Is Suppressed by 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase Inhibitors
    Article Snippet: .. To ascertain the binding specificity, 2.0 μL of a c‐jun gel supershift antibody (2 mg/mL, sc‐1694; Santa Cruz Biotechnology) per 6.0 μL of nuclear extract (10 μg protein) was added 30 minutes before addition of the labeled oligonucleotide probe and incubated for another 30 minutes at room temperature. .. Total RNA was isolated from cultured cells by solid phase extraction with the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions. cDNA was synthesized using the Omniscript RT Kit (Qiagen), according to the manufacturer's protocol.

    Article Title: Varicose Remodeling of Veins Is Suppressed by 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase Inhibitors
    Article Snippet: Preparation of nuclear extracts from the cultured HUVSMCs and subsequent nondenaturing 4% polyacrylamide gel electrophoresis were carried out, as described previously.15 The double-stranded gel shift oligodeoxynucleotide for AP-1 (50-GTGCTGACTCAGCAC-30) was end-labeled with [c-32P]ATP by using the 50-end labeling kit from Amersham GE Healthcare. .. The double-stranded AP-1 oligodeoxynucleotide was prepared from complementary single-stranded bonded oligodeoxynu- cleotides obtained from Biomers, as described prevously.16 To ascertain the binding specificity, 2.0 lL of a c-jun gel supershift antibody (2 mg/mL, sc-1694; Santa Cruz Biotechnology) per 6.0 lL of nuclear extract (10 lg protein) was added 30 minutes before addition of the labeled oligonucleotide probe and incubated for another 30 minutes at room temperature. .. Total RNA was isolated from cultured cells by solid phase extraction with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. cDNA was synthesized using the Omniscript RT Kit (Qiagen), according to the manufacturer’s protocol.

    Incubation:

    Article Title: Varicose Remodeling of Veins Is Suppressed by 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase Inhibitors
    Article Snippet: .. To ascertain the binding specificity, 2.0 μL of a c‐jun gel supershift antibody (2 mg/mL, sc‐1694; Santa Cruz Biotechnology) per 6.0 μL of nuclear extract (10 μg protein) was added 30 minutes before addition of the labeled oligonucleotide probe and incubated for another 30 minutes at room temperature. .. Total RNA was isolated from cultured cells by solid phase extraction with the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions. cDNA was synthesized using the Omniscript RT Kit (Qiagen), according to the manufacturer's protocol.

    Article Title: Varicose Remodeling of Veins Is Suppressed by 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase Inhibitors
    Article Snippet: Preparation of nuclear extracts from the cultured HUVSMCs and subsequent nondenaturing 4% polyacrylamide gel electrophoresis were carried out, as described previously.15 The double-stranded gel shift oligodeoxynucleotide for AP-1 (50-GTGCTGACTCAGCAC-30) was end-labeled with [c-32P]ATP by using the 50-end labeling kit from Amersham GE Healthcare. .. The double-stranded AP-1 oligodeoxynucleotide was prepared from complementary single-stranded bonded oligodeoxynu- cleotides obtained from Biomers, as described prevously.16 To ascertain the binding specificity, 2.0 lL of a c-jun gel supershift antibody (2 mg/mL, sc-1694; Santa Cruz Biotechnology) per 6.0 lL of nuclear extract (10 lg protein) was added 30 minutes before addition of the labeled oligonucleotide probe and incubated for another 30 minutes at room temperature. .. Total RNA was isolated from cultured cells by solid phase extraction with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. cDNA was synthesized using the Omniscript RT Kit (Qiagen), according to the manufacturer’s protocol.



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